Aspectos ultraestructurales de estadios preimaginales e imaginales de cuatro especies de phlebotominae (Diptera: Psychodidae) del estado Trujillo, Venezuela | Ultrastructural aspects of preimaginal and imaginal stages of four phlebotominae species (Diptera: Psychodidae) of Trujillo state, Venezuela)
Resumen
Ante el poder resolutivo limitado de la microscopía óptica, la Microscopía Electrónica de Barrido (MEB) aparece como una herramienta complementaria y útil para el estudio de la morfología de los integrantes de los Dípteros-psicodídeos de la subfamilia Phlebotominae. Por ello, en el presente trabajo se identificaron y describieron algunas características ultramorfológicas externas de huevos de Lutzomyia (Tricholateralis) gomezi; larvas estadio IV y pupas de Pintomyia (Pifanomyia) evansi; machos de Micropygomyia (Sauromyia) trinidadensis y hembras de Pi. (Pif.) youngi, obtenidas en condiciones de laboratorio a partir de hembras flebotominas capturadas en Trujillo, región andino-venezolana. El estudio ultramorfológico reveló que los huevos de Lu. (Trl.) gomezi poseen un patrón de esculpido exo-coriónico poligonal. Asimismo, se confirma que las larvas IV de Pi. (Pif.) evansi pueden colocarse en el Grupo cuatro de las especies flebotominas del Neotrópico que poseen antenas con segmento basal corto y el distal ovoide; mientras que la mayoría de las setas analizadas de sus pupas son simples y espiniformes, y las pre-alares largas, curvadas y delgadas con puntas afiladas. En varias regiones anatómicas de los imagos de Mi. (Sau.) trinidadensis y Pi. (Pif.) youngi se detectaron varios tipos de sensilas, incluyendo las escuamiformes, tricoideas, caéticas y microtriquias. Se discute la importancia de estos hallazgos ultraestructurales en el contexto de la taxonomía y sistema de quetotaxia de la subfamilia Phlebotominae, y de la posible función de las sensilas.
Palabras clave: Flebotominos, morfología, estadios inmaduros, adultos, Microscopía Electrónica de Barrido.
Abstract
Due to limitations in the resolution of optical microscopy, Scanning Electron Microscopy (SEM) appears to be considered as a useful complementary tool for the study on morphology of the Psychodid-Diptera subfamily Phlebotominae. Thus, in the present article some outer ultramorphological characteristics of laboratory reared eggs of Lutzomyia (Tricholateralis) gomezi; fourth instar larvae and puparium of Pintomyia (Pifanomyia) evansi; males of Micropygomyia (Sauromyia) trinidadensis and females of Pi. (Pif.) youngi of Trujillo, Venezuelan andes region, were identified and described. The ultramorphological study revealed that the exo-chorionic sculpturing pattern of the eggs of Lu. (Trl.) gomezi are polygonal. Likewise, it is confirmed that the fourth instar larvae of Pi. (Pif.) evansi can be placed in Group 4 of the Neotropical phlebotomine which posses’ antennae with a short basal segment and an ovoid distal segment; whereas most of its analyzed puparium setae are short, smooth and spinniforms, and the pre-alar ones long, curved, thin and sharp. Various types of sensilla, including squamiform, trichoid, chaetic and microtrichia ones were detected on several anatomic parts of adults of Mi. (Sau.) trinidadensis and Pi. (Pif.) youngi. The importance of these ultrastructural findings to phlebotomine sandfly taxonomy and chaetotaxy system and possible function of sensilla, is discussed.
Key words: Sandflies, morphology, inmature stages, adults, Scanning Electron Microscopy.
Referencias
ALMEIDA D, OLIVEIRA R, BRAZIL B, SOARES M. 2004. Patterns of exochorion ornaments on eggs of seven South American species of Lutzomyia sand lies (Diptera: Psychodidae). J. Med. Entomol. 41(5):819-825.
AÑEZ N, OVIEDO M. 1985. Two new larval diets for rearing sandflies in the laboratory. Trans. R. Soc. Trop. Med. Hyg. 79(5):739-740.
BARRETO M. 1941. Morfología dos ovos, larvas e pupas de algunos flebótomos de São Paulo. Ann. Fac. Med. Univ. São Paulo. 117(2):356-427.
BEJARANO E, PÉREZ-DORIA A, PATERNINA L, PATERNINA-GÓMEZ M, MARTÍNEZ L. 2012. Natural infection of Lutzomyia evansi (Diptera: Psychodidae) with Leishmania (Viannia) spp. in northern Colombia. Am. J. Trop. Med. Hyg. 87(Suppl.):173.
CAZORLA-PERFETTI D. 2015. Lista comentada de los flebotominos (Diptera: Psychodidae, Phlebotominae) citados para Venezuela. Saber. 27(2):178-231.
CAZORLA D, OVIEDO M. 1998. Quetotaxia del IV estadio larval de Lutzomyia youngi Feliciangeli & Murillo, 1987 (Diptera, Psychodidae, Phlebotominae). Bol. Dir. Malariol. San. Amb. 38(2):109-118.
CAZORLA D, OVIEDO M. 2015. Aspectos ultraestructurales de los estadios inmaduros de Micropygomyia (Sauromyia) trinidadensis (Diptera: Psychodidae, Phlebotominae). Saber. 27(2):232-241.
CAZORLA D, OVIEDO M, VIVENES M. 2010. Redescripción de la quetotaxia del cuarto estadio larval de Lutzomyia evansi (Diptera: Psychodidae, Phlebotominae). Rev. Colomb. Entomol. 36(1):76-81.
CONTRERAS M, VIVERO R, VÉLEZ I, PORTER C, URIBE S. 2014. DNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia. PLoS One. 9(1):e85496.
COSTA W, COSTA S, RANGEL E, SANTOS-MALLET J, SERRÃO J. 2012. Eggshell as a characteristic to identify Lutzomyia (Nyssomyia) intermedia (Lutz & Neiva, 1912) and Lutzomyia (Nyssomyia) neivai (Pinto, 1926) (Diptera: Psychodidae: Phlebotominae), vectors of cutaneous leishmaniasis. Rev. Pan-Amaz. Saúde. 3(1):19-24.
CHAIWONG T, SUKONTASON K, OLSON J, KURAHASHI H,CHAITHONG U, SUKONTASON K. 2008. Fine structure of the reproductive system of Chrysomya megacephala (Diptera: Calliphoridae): the external sexual organ. Parasitol. Res. 102(5):973-80.
DAYRAT B. 2005. Towards integrative taxonomy. Biol. J. Linn. Soc. 85:407-415.
DEPAQUIT J, GRANDADAM M, FOUQUE F, ANDRY P, PEYREFITTE C. 2010. Arthropod-borne viruses transmitted by phlebotomine sandflies in Europe: a review. Euro. Surveill. 15(10):pii=1950.
DUTARI L, LOAIZA J. 2014. American Cutaneous Leishmaniasis in Panama: a historical review of entomological studies on anthropophilic Lutzomyia sand fly species. Parasit. Vectors. 7:218.
ENDRIS R, YOUNG D, PERKINS P. 1987. Ultrastructural comparison of egg surface morphology of five Lutzomyia species (Diptera: Psychodidae). J. Med. Entomol. 24(4):412-415.
EWEL J, MADRIZ A, TOSI JR. J. 1976. Zonas de Vida de Venezuela. Memoria explicativa sobre el mapa ecológico. 2a ed. Editorial Sucre, Caracas, Venezuela, pp. 270.
FAUSTO A, MAROLI M, MAZZINI M. 1992. Ootaxonomy and eggshell ultrastructure of Phlebotomus sandflies. Med. Vet. Entomol. 6(3):201-208.
FAUSTO A, FELICIANGELI M, MAROLI M, MAZZINI M. 2001. Ootaxonomic investigation of five Lutzomyia species (Diptera, Psychodidae) from Venezuela. Mem. Inst. Oswaldo Cruz. 96(2):197-204.
FELICIANGELI M. 2014. Leishmaniasis en Venezuela: situación actual, acciones y perspectivas para el control vectorial en el marco de un programa de control multisectorial. Bol. Mal. Salud Amb. 54(1):1-7.
FELICIANGELI M, CASTEJON O, LIMONGI J. 1993. Egg surface ultrastructure of eight New World phlebotomine sand fly species (Diptera: Psychodidae). J. Med. Entomol. 30(4):651-656.
FERNANDES F, LINARDI P, CHIARINI-GARCIA H. 2002. Morphology of the antenna of Dermatobia hominis (Diptera: Cuterebridae) based on scanning electron microscopy. J. Med. Entomol. 39(1):36-43.
FERNANDES F DE F, BAHIA-NASCIMENTO A, PINTO L, LEAL C DE S, SECUNDINO N, PIMENTA P. 2008. Fine structure and distribution pattern of antennal sensilla of Lutzomyia longipalpis (Diptera: Psychodidae) sand flies. J. Med. Entomol. 45(6):982-990.
FORATTINI O. 1973. Subfamilia Phlebotominae-morfología e classificação. En: Entomología médica. IV.- Psychodidae. Phlebotominae. Leishmanioses. Bartonelose. Ed. Edgar Blucher, São Paulo, Brasil, pp. 66-118.
GALATI E. 2012. Phlebotominae (Diptera, Psychodidae): Classificação, morfologia, terminologia e identificação de adultos. Apostila da Disciplina HEP 5752- Bioecologia e identificação de Phlebotominae. Universidade de São Paulo, Faculdade de Saúde Pública, São Paulo, Brasil, pp. 121.
GHOSH N, MUKHOPADHYAY J. 1996. A comparison of chorionic sculpturing of four Indian Phlebotomine sandflies (Diptera: Psychodidae) by scanning electron microscopy. Parasite. 3(1):61-67.
HALLBERG E, HANSSON B. 1999. Arthropod sensilla: morphology and phylogenetic considerations. Microsc. Res. Tech. 47(6):428-39.
HANSON W. 1968. The immature stages of the subfamily Phlebotominae in Panama (Diptera, Psychodidae). Kansas, USA: University of Kansas, Deparment of Entomology. [Dissertation Doctor of Philosophy], pp. 104.
HINTON H. 1969. Respiratory systems of insect eggs shell. Annu. Rev. Entomol. 14:343-368.
LEITE A, WILLIAMS P. 1996. Description of the fourth instar larva of Lutzomyia longipalpis, under scanning electron microscopy. Mem. Inst. Oswaldo Cruz. 91(5):571-578.
LEITE A, WILLIAMS P. 1997. The first instar larva of Lutzomyia longipalpis (Diptera: Phlebotomidae). Mem. Inst. Oswaldo Cruz. 92(2):197-203.
LEITE A, WILLIAMS P, DOS SANTOS M. 1991. The pupa of Lutzomyia longipalpis (Diptera: Psychodidae-Phlebotominae). Parassitologia. 33(Suppl.1):477-484.
MARCONDES C. 2007. A proposal of generic and subgeneric abbreviations for phlebotomine sandflies (Diptera: Phlebotominae) of the world. Entomol. News. 188(4):351-356.
MCIVER S. 1982. Sensilla of mosquitoes (Diptera: Culicidae). J. Med.Entomol. 19(5):489-535.
MINNICK M, ANDERSON B, LIMA A, BATTISTI J, LAWYER P, BIRTLES R. 2014. Oroya fever and verruga peruana: bartonelloses unique to South America. PLoS Negl. Trop. Dis. 8(7):e2919.
MITCHELL B, ITAGAKI H, RIVET M. 1999. Peripheral and central structures involved in insect gustation. Microsc. Res. Tech. 47(6):401-415.
MONTOYA-LERMA J. 1996. The biology of visceral leishmaniasis vectors in the San Andrés de Sotavento focus, Colombia. London, England: University of London, Faculty of Science [PhD Thesis], pp.189.
MUKHOPADHYAY J, GHOSH K. 1997. Diagnostic importance of female external genital structure of phlebotomine sand flies (Diptera: Psychodidae) as observed by scanning electron microscopy. Mem. Inst. Oswaldo Cruz. 92(1):57-61.
NIEVES E, ORAÁ L, RONDÓN Y, SÁNCHEZ M, SÁNCHEZ Y, RUJANO M, RONDÓN M, ROJAS M, GONZÁLEZ N, CAZORLA D. 2014. Riesgo de transmisión de Leishmania (Kinetoplastida: Trypanosomatidae) en Mérida, Venezuela. Avan. Biomed. 3(2):57-64.
NGERN-KLUN R, SUKONTASON K, METHANITIKORN R,VOGTSBERGER R, SUKONTASON K. 2007. Fine structure of Chrysomya nigripes (Diptera: Calliphoridae), a fly species of medical importance. Parasitol. Res. 100(5):993-1002.
NOGUERA P, CHAVES L, NIEVES E. 2003. Effects of blood ingestion on patterns on the chorion of eggs of Lutzomyia ovallesi (Diptera: Psychodidae). Parasitol. Latinoam. 58(1-2):49-53.
OCA-AGUILAR A, REBOLLAR-TÉLLEZ E, IBÁÑEZ-BERNAL S. 2014. Descriptions of the immature stages of Dampfomyia (Coromyia) beltrani (Vargas y Díaz-Nájera) (Diptera: Psychodidae), with notes on morphology and chaetotaxy nomenclature. Zootaxa. 3887(3):251-297.
OCA-AGUILAR A, REBOLLAR-TÉLLEZ E, IBÁÑEZ-BERNAL S. 2016. The immature stages of Micropygomyia (Coquillettimyia) chiapanensis (Dampf) (Diptera: Psychodidae, Phlebotominae). Zootaxa. 4105(5):455-482.
PAPPAS L, LARSEN J. 1976. Gustatory hairs on the mosquito, Culiseta inornata. J. Exp. Zool. 196(5):351-360.
PÉREZ E, OGUSUKU E. 1997. Chorion patterns on eggs of Lutzomyia sand flies from the Peruvian Andes. Med.Vet. Entomol. 11(2):127-133.
PESSOA F, QUEIROZ R, WARD R. 2001. External morphology of sensory structures of fourth instar larvae of Neotropical species of Phlebotomine sand flies (Diptera: Psychodidae) under Scanning Electron Microscopy. Mem. Inst. Oswaldo Cruz. 96(8):1103-1108.
READY P. 2013. Biology of phlebotomine sand flies as vectors of disease agents. Annu. Rev. Entomol. 58:227-250.
ROGO L, KOKWARO E, MUTINGA M, KHAMALA C. 1992. Differentiation of vector species of phlebotominae (Diptera: Psychodidae) in Kenya by chorionic sculpturing of their eggs. J. Med. Entomol. 29(6):1042-1044.
ROJAS E, SCORZA J, MORALES G, MORALES C, BARAZARTE R, TORRES A. 2004. Diversity and species composition of sand flies (Diptera: Psychodidae) in a Venezuelan urban focus of cutaneous leishmaniasis. J. Am. Mosq. Control Assoc. 20(2):189-194.
SCHLICK-STEINER B, STEINER F, SEIFERT B, STAUFFER C, CHRISTIAN E, CROZIER R. 2010. Integrative taxonomy: a multisource approach to exploring biodiversity. Annu. Rev. Entomol. 55:421-438.
SCORZA J, ORTIZ I, GOMEZ I. 1968. Observaciones biológicas sobre algunos flebotomos de Rancho Grande (Venezuela). Sobre los factores microclimáticos que determinan la endemicidad de la flebotomofauna de Rancho Grande. Acta Biol. Venez. 6(2):76-83.
SEENIVASAGAN T, SHARMA K, SHRIVASTAVA A, PARASHAR B, PANT S, PRAKASH S. 2009. Surface morphology and morphometric analysis of sensilla of Asian tiger mosquito, Aedes albopictus (Skuse): an SEM investigation. J. Vector Borne Dis. 46:125-135.
SIERRA D, VELEZ I, URIBE S. 1995. Electronic microscopy of eggs as a taxonomic parameter. Bol. Dir. Mal. San. Amb. 30(Supl. 1):327-336.
SIERRA D, VÉLEZ I, URIBE S. 2000. Identificación de Lutzomyia spp. (Diptera: Psychodidae) grupo verrucarum por medio de microscopía electrónica de sus huevos. Rev. Biol. Trop. 48(2-3):615-622.
SPIEGEL C, BRAZIL R, SOARES M. 2000. Sensilla on the terminalia of Lutzomyia spp. (Diptera: Psychodidae) sand flies. J. Med. Entomol. 37(6):860-863.
SPIEGEL C, OLIVEIRA S, BRAZIL R, SOARES M. 2005. Structure and distribution of sensilla on maxillary palps and labella of Lutzomyia longipalpis (Diptera: Psychodidae) sand flies. Microsc. Res. Tech. 66(6):321-330.
VALDERRAMA A, TAVARES M, ANDRADE D. 2014. Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus. Parasit. Vectors. 7:9.
VALDIVIA H, DE LOS SANTOS M, FERNANDEZ R, BALDEVIANO G, ZORRILLA V, VERA H, LUCAS C, EDGEL K, LESCANO A, MUNDAL K, GRAF P. 2012. Natural Leishmania infection of Lutzomyia auraensis in Madre de Dios, Peru, detected by a fluorescence resonance energy transfer-based real-time polymerase chain reaction. Am. J. Trop. Med. Hyg. 87(3):511-517.
VIVERO R, TORRES C, BEJARANO E, CADENA H, ESTRADA G, FLOREZ F, ORTEGA E, APARICIO Y, MUSKUS C. 2015. Study on natural breeding sites of sand flies (Diptera: Phlebotominae) in areas of Leishmania transmission in Colombia. Parasit. Vectors. 8:116.
WARD R. 1976. The immature stages of some phlebotomine sandflies from Brazil (Diptera: Psychodidae). Syst. Entomol. 1(3):227-240.
WARD R, READY P. 1975. Chorionic sculpturing in some sandfly eggs (Diptera, Psychodidae). J. Entomol. 50(2):127-134.
WHO (WORLD HEALTH ORGANIZATION). 2010. Control of the leishmanioses. Technical Report Series 949, Ginebra, Suiza, pp. 186.
YOUNG D, DUNCAN M. 1994. Guide to the identification and geographic distribution of Lutzomyia sand flies in México, the West Indies, Central and South America (Diptera: Psychodidae). Memories of the American Entomological Institute, Number 54. Associated Publishers, Gainesville, Florida, USA, pp. 881.
ZACHARUK R. 1980. Ultrastructure and function of insect chemosensilla. Annu. Rev. Entomol. 25:27-47.
ZIMMERMAN J, NEWSON H, HOOPER G, CHRISTENSEN H. 1977. A comparison of the egg surface structure of six anthropophilic phlebotomine sand flies (Lutzomyia) with the scanning electron microscope (Diptera: Psychodidae). J. Med. Entomol. 13(4-5):574-579.
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